The Scientific World Journal

Research Article

Effect of Dietary Combination of Methionine and Fish Oil on Cellular Immunity and Plasma Fatty Acids in Infectious Bursal Disease Challenged Chickens

Table 2

Fatty acid compositions of treatment diets.


	Fatty acid (%)	Starter (1–21 d)						Finisher (21–42 d)
	Fatty acid (%)	M₁F₀	M₂F₀					M₁F₀	M₂F₀

Myristic	C14:0	0.81	0.73	1.05	1.05	2.61	2.75	0.84	1.25	2.20	1.30	2.44	2.62
Pentadecanoic	C15:0	0.04	0.05	0.33	0.33	0.86	0.91	0.27	0.10	0.59	0.38	0.80	0.83
Palmitic	C16:0	30.84	27.94	14.13	13.94	22.00	23.33	26.92	27.82	18.08	16.67	20.80	22.13
Palmitoleic	C16:1	0.17	0.18	1.27	1.27	3.09	3.21	0.20	0.22	2.20	1.37	2.82	3.02
Heptadecanoic	C17:0	0.12	0.1	0.80	0.81	1.97	2.01	0.09	0.05	1.28	0.81	1.80	1.84
Stearic	C18:0	3.69	3.33	4.34	4.34	5.82	6.31	3.09	3.05	4.52	4.47	5.54	5.92
Oleic	C18:1 n-9	36.42	36.28	24.39	25.33	21.04	22.07	34.87	35.88	22.45	27.36	20.10	20.57
Linoleic	C18:2 n-6	26.70	27.79	45.11	44.16	25.38	24.58	31.97	29.88	38.16	39.10	25.03	25.60
-Linolenic.	C18:3 n-3	0.61	0.59	0.21	0.22	0.27	0.27	0.73	0.67	0.34	0.39	0.43	0.48
Eicosapentaenoic (EPA)	C20:5 n-3	ND	ND	0.06	0.05	0.10	0.21	ND	ND	2.29	2.02	1.96	1.96
Docosahexaenoic (DHA)	C22:6 n-3	ND	ND	7.33	7.29	14.77	13.34	ND	ND	3.92	4.17	15.09	13.98

Total SFA		35.97	33.61	20.71	20.75	33.34	35.39	31.88	33.18	28.29	23.95	31.58	33.54
Total UFA		64.03	66.39	79.29	79.25	66.66	64.61	68.12	66.82	71.71	76.05	68.42	66.46
Total MUFA		36.72	38	26.05	26.99	25.05	26.23	35.43	36.27	25.33	29.16	23.76	24.50
Total PUFA n-3		0.61	0.59	8.14	8.10	15.93	14.79	0.73	0.67	8.02	7.79	18.63	16.86
Total PUFA n-6		26.70	27.79	45.11	44.16	25.38	24.58	31.97	29.88	38.16	39.10	25.03	25.60
UFA/SFA		1.78	2.07	3.83	3.82	2.00	1.83	2.14	2.02	2.78	3.18	2.17	1.99
PUFA/SFA		0.76	0.9	2.57	2.52	1.25	1.08	1.03	0.93	1.83	1.96	1.41	1.26
n-6/n-3		43.77	47.10	5.54	5.45	1.59	1.66	43.79	44.59	4.76	5.02	1.34	1.52

SFA: saturated fatty acid; UFA: unsaturated fatty acid; MUFA: monounsaturated fatty acid; PUFA: polyunsaturated fatty acid.
Total saturated: sum of 8:0, 10:0, 12:0, 14:0, 15:0, 16:0, 17:0, and 18:0.
Total unsaturated: sum of 15:1, 16:1, 17:1, 18:1, 18:2 n-6, 18:3 n-3, 20:4 n-6, 20:5 n-3, 22:5 n-3, and 22:6 n-3.
Total MUFA: sum of 15:1, 16:1, 17:1, and 18:1.
Total PUFA n-3: sum of 18:3 n-3, 20:5 n-3, 22:5 n-3, and 22:6 n-3. Total PUFA n-6: sum of 18:2 n-6 and 20:4 n-6.
F₀: 0% fish oil; : 2.5% fish oil; : 5.5% fish oil.
M₁: methionine (NRC level); M₂: methionine (2 fold of NRC).