Effect of Dietary Combination of Methionine and Fish Oil on Cellular Immunity and Plasma Fatty Acids in Infectious Bursal Disease Challenged Chickens

Table 3

Effect of fish oil and methionine supplementation on plasma fatty acids composition of 42-day-old broiler chickens (%).

	18:3 n-3	20:5 n-3	22:6 n-3	18:2 n-6	20:4 n-6	Total; n-3 PUFA	Total; n-6 UFA	n-6/n-3	PUFA/SFA
F0	0.63±0.08	0.61±0.24^c	1.43±0.51^c	25.54±0.86^a	8.45±0.44^a	3.30±0.74^c	33.79±0.83^a	9.90±0.39^a	0.90±0.042^b
F2.5	0.82±0.11	3.71±0.31^b	12.60±0.67^b	25.43±1.04^a	5.26±0.55^b	17.89±0.92^b	30.34±1.07^a	1.78±0.50^b	1.26±0.54^a
F5.5	0.92±0.10	5.53±0.28^a	17.65±0.61^a	17.09±0.94^b	4.90±0.51^b	25.87±0.81^a	22.25±0.98^b	0.89±0.46^b	1.13±0.49^a
M1	0.59±0.05	0.67±0.16	1.37±0.13	24.38±1.16	7.75±0.65	3.55±0.25	32.36±1.06	9.65±0.60	0.85±0.03
M2	0.68±0.07	0.56±0.29	1.30±0.19	26.65±1.40	8.74±0.93	3.59±0.27	35.73±1.50	10.56±0.96	0.95±0.05
ANOVA (𝑃 value)
M	0.65	0.056	0.57	0.97	0.79	0.37	0.66	0.71	0.29
F	0.09	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001
M × F	0.62	0.09	0.81	0.24	0.95	0.38	0.51	0.96	0.47

a--cMeans ± SEM within a column subgroup with no common letters differ at 𝑃<0.05.
F₀: 0% fish oil; F_2.5: 2.5% fish oil; F_5.5: 5.5% fish oil.
M₁: methionine (NRC level); M₂: methionine (2-fold of NRC).