Effect of Dietary Combination of Methionine and Fish Oil on Cellular Immunity and Plasma Fatty Acids in Infectious Bursal Disease Challenged Chickens

Table 4

Effect of fish oil and methionine supplementation on blood parameters in broiler chickens challenged with IBD.

Before challenge

7 d after challenge

14 d after challenge

WBC; 10^9/L

ALB; (g/L)

TP; (g/L)

GLU; (g/L)

WBC; 10^9/L

ALB; (g/L)

TP; (g/L)

GLU; (g/L)

WBC; 10^9/L

ALB; (g/L)

TP; (g/L)

GLU; (g/L)

F0

17.6±1.7^c

11.7±0.7

23.1±1.1

11.4±0.9^b

40.4±6.6^b

10.3±1.0

22.5±1.2^b

10.5±1.2

49.0±5.5

12.2±1.2

28.1±1.4

19.0±1.7

F2.5

32.4±1.7^b

12.4±0.7

21.0±1.1

9.0±0.8^b

72.8±7.4^b

10.5±1.0

23.3±1.1^b

10.8±1.0

54.6±6.2

14.9±1.1

32.8±1.2

19.4±1.4

F5.5

40.2±2.0^a

10.0±0.7

23.4±1.1

15.9±1.0^a

80.4±8.0^a

12.4±0.9

25.8±1.1^a

12.4±0.9

54.5±6.3

12.4±1.2

29.3±1.3

16.9±1.6

M1

31.8±1.5

10.3±0.5

23.7±0.9

12.7±0.7

55.3±6.2

11.8±0.8

21.7±0.9

9.3±0.9^b

48.4±5.1

13.8±0.9

29.0±1.1

18.4±1.3

M2

28.8±1.5

11.9±0.5

22.8±0.9

12.3±0.8

68.9±5.8

10.5±0.8

23.9±0.9

13.8±0.8^a

59.2±7.1

12.1±1.0

29.5±1.0

18.3±1.2

ANOVA (𝑃 value)

F

0.0001

0.07

0.32

0.003

0.002

0.22

0.04

0.01

0.96

0.27

0.98

0.85

M

0.15

0.09

0.49

0.99

0.18

0.45

0.12

0.26

0.35

0.24

0.12

0.44

F × M

0.14

0.97

0.97

0.11

0.27

0.61

0.52

0.36

0.54

0.51

0.30

0.16

a--cMeans ± SEM within a column subgroup with no common letters differ at 𝑃<0.05.
WBC: total white blood cell; TP: total protein; ALB: albumin; GLU: globulin.
F₀: 0% fish oil; F_2.5: 2.5% fish oil; F_5.5: 5.5% fish oil.
M₁: methionine (NRC level); M₂: methionine (2-fold of NRC).