Effect of Dietary Combination of Methionine and Fish Oil on Cellular Immunity and Plasma Fatty Acids in Infectious Bursal Disease Challenged Chickens

Table 5

Effect of fish oil and methionine supplementation on liver enzymes (U/L) in broiler chickens challenged with IBD.

	Before challenge		14 d after challenge
	ALT	AST	ALT	AST
F0	15.5±2.8	246±10	3.8±0.7	354±30
F2.5	23.0±3.5	260±10	2.1±0.5	361±23
F5.5	21.2±3.3	272±10	3.6±0.6	293±27
M1	21.7±2.1	272±8	2.8±0.6	337±23
M2	18.8±2.3	274±8	3.6±0.5	336±20
ANOVA (𝑃 value)
F	0.21	0.19	0.48	0.16
M	0.36	0.30	0.24	0.96
F × M	0.75	0.31	0.98	0.46

ALT: alanine aminotransferase; AST: aspartate aminotransferase.
F₀: 0% fish oil; F_2.5: 2.5% fish oil; F_5.5: 5.5% fish oil.
M₁: methionine (NRC level); M₂: methionine (2-fold of NRC).