Effect of Dietary Combination of Methionine and Fish Oil on Cellular Immunity and Plasma Fatty Acids in Infectious Bursal Disease Challenged Chickens

Table 7

Effect of fish oil and methionine supplementation on serum IL-2 and IFN-𝛾 levels (pg/mL) in broiler chickens challenged with IBD.

	IL-2			IFN-𝛾
	Before challenge	2 d after challenge	7 d after challenge	Before challenge	2 d after challenge	7 d after challenge
F0	0.452±0.008	0.365±0.002	0.428±0.002^b	0.99±0.07	1.38±0.05	1.10±0.04^a
F2.5	0.442±0.009	0.365±0.003	0.423±0.002^b	0.98±0.07	1.35±0.05	0.87±0.04^b
F5.5	0.438±0.008	0.374±0.002	0.447±0.002^a	0.95±0.06	1.39±0.05	0.92±0.04^b
M1	0.440±0.007	0.373±0.002^a	0.435±0.002	1.06±0.06	1.37±0.04	0.93±0.03^b
M2	0.448±0.007	0.361±0.001^b	0.432±0.001	0.95±0.05	1.37±0.04	1.06±0.03^a
ANOVA (P value)
F	0.57	0.051	0.006	0.88	0.76	0.002
M	0.41	0.008	0.54	0.49	1.00	0.04
F × M	0.18	0.014	0.10	0.26	0.14	0.01

a--cMeans ± SEM within a column subgroup with no common letters differ at 𝑃<0.05.
F₀: 0% fish oil; F_2.5: 2.5% fish oil; F_5.5: 5.5% fish oil.
M₁: methionine (NRC level); M₂: methionine (2-fold of NRC).