Research Article
Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran
Table 1
Primers, PCR conditions, and restriction enzymes used for genotyping TLRs genes.
| SNPs product | Primer sequence 5′-3′ | AT
| PCR product
| RE |
| TLR4 | | | | | Arg299Gly | F: GATTAGCATACTTAGACTACTACCTCCATG | 60° | 249 bp | NcoI | R: GATCAACTTCTGAAAAAGCATTCCCAC | Thr399Ile | F: GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA | 61° | 406 bp | HinfI | R: CCTGAAGACTGGAGAGTGAGTTAAATGCT |
| TLR9 | | | | | T-1486C | F: TTCATTCAGCCTTCACTCAG | 55° | 558 bp | BslI | R: TCAAAGCCACAGTCCACAG |
|
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F: forward primer; R: reverse primer; AT: annealing temperature; RE: restriction enzyme.
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