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The Scientific World Journal
Volume 2013, Article ID 536369, 6 pages
http://dx.doi.org/10.1155/2013/536369
Research Article

Application of a Developed Method for the Extraction of Triazines in Surface Waters and Storage Prior to Analysis to Seawaters of Galicia (Northwest Spain)

Department of Analytical Chemistry, Faculty of Science, A Coruña University, Campus da Zapateira, Rua da Fraga 10, 15008 A Coruña, Spain

Received 28 August 2013; Accepted 17 September 2013

Academic Editors: H. A. Alegria and R. A. Smith

Copyright © 2013 Noelia Rodríguez-González et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple method based on solid-phase extraction combined with liquid chromatography for simultaneous determination of nine triazine herbicides (ametryn, atrazine, cyanazine, prometryn, propazine, simazine, simetryn, terbuthylazine, and terbutryn) in surface water samples was developed and validated. Under optimized conditions, 50 mL of water sample was pumped through the Oasis HLB cartridge, and triazines were eluted with 3 mL acetone. Finally the extract was concentrated to dryness, reconstituted with 1 mL methanol : water (1 : 1) and injected into the HPLC-DAD system. The stability of the herbicides on the cartridges at −18 and 4°C was also evaluated, and the recoveries obtained after three weeks of storage were satisfactory for all compounds. The analytical features of the proposed method were satisfactory: repeatability and intermediate precision were <10% and recoveries in spiked river water and seawater samples were higher than 93% for all compounds studied. Limits of quantification (varied from 0.46 to 0.98 µg L−1) were adequately allowing the determination of these compounds at the levels requested by the 2008/105/EC Directive. Finally, this method was applied to the analysis of 50 seawater samples from Galicia (northwest Spain).