Human SLC26A1 Gene Variants: A Pilot Study

Table 1

Primers used for PCR and DNA sequencing.

Primer	^aDirection	Sequence (5′ to 3′)	^bPCR fragment
Primers used for PCR
P1	F	GCTGAGGCAGGAGAATGGCGTGAAC	1
P2	R	CTGGGGTCCAGGTGTGTGGAATGG	1
P3	F	CAGCTTGGACCAGGCTGGCTCCTTG	2
P4	R	GGATTGGTGCTGGGCCTTCTCCT	2
P5	F	GTAATCCCATCTCACCTCACGATG	3
P6	R	CATCGAGCAGTGGCTGTGAGAGGTAG	3
P7	F	GATGCTCACGTGGATGTCACAGTTG	4
P8	R	CGAACTCTGTGGCATCCTCGTAGAAG	4
P9	F	CACCTGTATGCTGGTCAGCACAGAG	5
P10	R	TGTCCCATTCCTTCCACCTAGAG	5
Primers used for sequencing
P11	F	CCAGGAGGCAGAGCTTCCAG	1
P12	F	GCATATGCACGCACCGGCAGCCTTG	1
P13	R	AGCCTGAGGGTGCCCCTAAGAAACC	1
P14	R	CAAGGAGCCAGCCTGGTCCAAGCTG	1
P15	F	CTGGCTCCTTG CAGGACCAG	2
P16	F	CCATTCCACACACCTGGACCCCAG	2
P17	R	CTTCTCAGCGACGTTAGGCAAAGAC	2
P18	R	CATCGTGAGGTGAGATGGGATTAC	2
P19	F	AGGAGAAGGCCCAGCACCAATCC	3
P20	F	CAAGCACAGGGTTGGCAGAGGAGGTG	3
P21	R	CCTCACCAGCACCTGGCATGG	3
P22	R	TGCCCAAGCCTTGCTGTCTTG	3
P23	F	TTGTCAGGTCCTGTGCCGTGAC	4
P24	R	CTCTGTGCTGACCAGCATACAGGTG	4
P25	F	CTTCTACGAGGATGCCACAGAGTTCG	5
P25	F	ATCACACGCAGGACCCAAACACTCAG	5
P26	R	ATTCCTTCCACCTAGAGCTGAGG	5
P27	R	CTGAGTGTTTGGGTCCTGCGTGTGAT	5

^aF: forward primer; R: reverse primer. ^bPCR fragment denoted in Figure 1(a).