The Value of Routine Polymerase Chain Reaction Analysis of Intraocular Fluid Specimens in the Diagnosis of Infectious Posterior Uveitis

Table 6

Estimated sensitivity, specificity, positive predictive value, and negative predictive value for each pathogen tested and broken down into AC and vitreous samples.

	Sample number	True +ve		False +ve		True −ve		False −ve		Sens	Spec	PPV	NPV
CMV	154	69	45%	1	1%	74	48%	7	5%	91%	99%	99%	91%
AC	45	13	29%	0	0%	31	69%	1	2%	93%	100%	100%	97%
V	106	56	53%	1	1%	43	41%	6	6%	90%	98%	98%	88%
NS	3	2	67%	0	0%	1	33%	0	0%	—	—	—	—
VZV	148	15	10%	2	1%	126	85%	5	3%	75%	98%	88%	96%
AC	43	1	2%	0	0%	39	91%	3	7%	25%	100%	100%	93%
V	102	14	14%	2	2%	84	82%	2	2%	88%	98%	88%	98%
NS	3	0	0%	0	0%	3	100%	0	0%	—	—	—	—
TOXO	150	12	8%	0	0%	134	89%	4	3%	75%	100%	100%	97%
AC	45	1	2%	0	0%	43	96%	1	2%	50%	100%	100%	98%
V	102	11	11%	0	0%	88	86%	3	3%	79%	100%	100%	97%
NS	3	0	0%	0	0%	3	100%	0	0%	—	—	—	—
MTB	43	1	2%	0	0%	39	91%	3	7%	25%	100%	100%	93%
AC	20	1	5%	0	0%	16	80%	3	15%	25%	100%	100%	84%
V	22	0	0%	0	0%	22	100%	0	0%	0%	—	—	100%
NS	1	0	0%	0	0%	1	100%	0	0%	—	—	—	—
Total	495	97	20%	3	1%	373	75%	19	4%	84%	99%	97%	95%

AC: anterior chamber; V: vitreous; NS: not specified; Sens: sensitivity; Spec: specificity; PPV: positive predictive value; NPV: negative predictive value.
(1) Three patients had samples taken from an unknown site (not specified in the clinical notes).
(2) Simultaneous vitreous and aqueous sampling was performed on 4 patients.
(3) Two patients had repeat sampling (both were vitreous sample repeats).