Sponge-Like: A New Protocol for Preparing Bacterial Ghosts

Table 1

Plackett-Burman experimental design.

Exp. no.	Variables					Basic Experiment	Basic Experiment	H2O2	H2O2	Ethanol	Ethanol	Response
	SDS	H2O2	CaCO3	NaOH	Shaking rate/Temperature	DNA µg/mL	Protein µg/mL	DNA µg/mL	Protein µg/mL	DNA µg/mL	Protein µg/mL	BGQ%
1	1	1	1	−1	1	164.5	3425.445	20.2	353.547	8.1	45.477	100
2	−1	−1	−1	−1	−1	136.2	2400.012	16.45	432.765	11.5	500.247	90
3	1	−1	1	1	−1	178.6	3137.913	0.6	0	16.55	391.689	10
4	−1	−1	−1	1	1	74.2	2589.255	4.4	151.101	5.65	123.228	30
5	−1	1	1	1	−1	121.15	2078.739	19.25	337.41	9.7	38.142	50
6	1	1	−1	1	−1	99.85	3082.167	16.1	222.984	5.4	233.253	60
7	−1	1	−1	−1	−1	106.1	1880.694	33	651.348	4.75	126.162	90
8	−1	−1	1	1	1	146.9	2400.012	33	284.598	7.7	29.34	0
9	1	1	−1	1	1	0	0	5.15	968.22	123.6	4.401	0
10	−1	1	1	−1	1	90.15	1805.877	13.1	1043.037	6.8	36.675	80
11	1	−1	−1	−1	1	122.2	2100.744	12.05	355.014	5.05	36.675	100
12	1	−1	1	−1	−1	59.4	2565.783	8.15	234.72	6.75	365.283	60