Research Article

Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children

Table 5

Amino acid changes in the P region.

SubjectSpacer domainReverse transcriptase domain
B-cell epitope aa 225–250Region outside the B-cell epitopeT-cell epitope
aa 412–427,
aa 426–435,
aa 455–463,
aa 501–516
Region outside the T-cell epitopeYMDD

Y1E228AS202P, V257M, T265I, P290S, R315Q, C340S Wild typeW499R, M553VWild-type
Y2P227S, E228AS202P, S262F, P264H, P290S, R315Q, P319T Wild typeW499R, M553VWild-type
Y3P227S, E228AS202P, S262F, P264H, P290S, R315Q, P319T, E336KWild typeW499R, M553VWild-type
Y4P227S, E228AS202P, S262F, P264H, P290S, R315Q, P319TWild typeW499R, M553VWild-type
Y5V232L, S234R, T236K, I242S, L243I, S249PT190K, I214V, F218L, Q253R, S254T, T269A, A272T, R294G, Q305H, H306N, S314P, L321F, S340TWild typeL437I Wild-type

GenBank number D00330 (genotype Ba/serotype adw) was used as the reference sequence for Y1–Y4. GenBank number AF223958 (genotype C/serotype adr) was used as the reference sequence for Y5.