DNA Damage Sensor γ-H2AX Is Increased in Preneoplastic Lesions of Hepatocellular Carcinoma
Figure 1
γ-H2AX is expressed at different levels in HCC. (a) Immunofluorescence staining shows that phosphorylated histone H2AX (γ-H2AX) is located in the nuclei of HCC cells (green) (original magnification ×100). Case 2: HCC with increased γ-H2AX expression; Case 7: HCC with sparse expression of γ-H2AX. DAPI (blue): nucleus counterstain. (b) Representative data of western blotting for γ-H2AX in liver tissues. H1 and H2: healthy livers; Cases 2 and 7: HCC cases. T: tumor tissues; N: adjacent nontumorous liver tissues. (c) Immunohistochemical staining of γ-H2AX. Cases 1 and 7: HCC cases with negative to low expression of γ-H2AX. Cases 2 and 11: HCCs with high expression of γ-H2AX (original magnification ×40). Arrows indicate positive staining in the nuclei.
