Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-Producing Escherichia coli Infection
Quantification of biofilm formation of STEC strains by crystal violet (CV) staining expressed in biofilm biomass units (BBU): (a) in TSB; with addition of 0.5% glucose; in thioglycollate medium alone and with the addition of 0.5% glucose. (b) ROS ratio (ROS/BBU) determined by NBT assay. (c) NO and BBU ratio (NO/BBU) determined by Griess method. Error bars represent the standard deviations of the means of three independent experiments. versus and versus thioglycollate being considered statistically significant.