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The Scientific World Journal
Volume 2013 (2013), Article ID 608650, 6 pages
Research Article

A Comparison of Ice Cold Water Pretreatment and α-Bromonaphthalene Cytogenetic Method for Identification of Papaver Species

1Department of Field Crops, Faculty of Agriculture, Ankara University, 06110 Diskapi, Turkey
2Department of Agricultural Biotechnology, Faculty of Agriculture, Namik Kemal University, 59030 Tekirdag, Turkey

Received 18 May 2013; Accepted 7 August 2013

Academic Editors: K. M. Khawar, S. Onarici, and C. A. Ozel

Copyright © 2013 Amir Rezaei Osalou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The plants belonging to many species in genus Papaver are very similar and have very small chromosomes that make identification very difficult. The study aimed to compare the effects of α-bromonaphtalene and ice cold water pretreatment to identify chromosomes of Papaver species collected from different areas of Iranian West Azerbaijan and Turkish Van, Agri and, Hakkari provinces. The seeds were germinated in Jacobson trays at 24°C under continuous light. Thereafter, roots from 1.5 cm long plantlets were pretreated with α bromonaphtalene for 15, 30, and 45 min or at 0°C in ice cold water for 24 h before fixing, hydrolyzation, and feulgen staining. The ice cold water pretreatment was more appropriate and easy to determine chromosomes. Seeds from seven samples did not germinate. Sixty samples out of the rest of 62 samples were identified as P. pseudo orientale, one sample was identified as P. bracteatum, and another as P. orientale. This is the first study that used ice cold water to determine the chromosomes in papaver species. It is hoped that it will also facilitate to determine chromosome number and identify other papver species.