Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues

Figure 3

Propidium iodide staining and 3D confocal imaging of the biopsied intestinal tissues with acute rejection. ((a) and (b)) Optically cleared intestinal tissues without (a) or with (b) acute rejection. ((c) and (d)) Propidium iodide fluorescence signals of the intestinal tissues without (c) or with (d) acute rejection. ((e) and (f)) Histopathology of the intestinal tissues without (e) or with (f) acute rejection (H and E stain, 100x). Bar: 100 μm. Arrow indicated residual crypts in the intestinal tissues with acute rejection.