Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues

<table>PGP9.5 staining of the myenteric plexus and nerve fibers in the donor and recipient intestinal tissues from a MMIHS patient that received intestinal transplantation. (a) Intestinal tissues in PBS. (b) Optically cleared intestinal tissues. ((c) and (d)) The permeabilized donor (c) and recipient (d) intestinal tissues were stained with PGP9.5 prior to FocusClear treatment. ((e) and (f)) PGP9.5 immunohistochemical studies of the intestinal tissues ((e), donor; (f), recipient, 100x). CM: circular muscle layer; MP: myenteric plexus; LM: longitudinal muscle layer. Bar: 100 <i>μ</i>m.</table>

The Scientific World Journal

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Figure 5

Figure 5: Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues 