Development of a pyrG Mutant of Aspergillus oryzae Strain S1 as a Host for the Production of Heterologous Proteins

Figure 4

Gel electrophoretic profile of the (a) (lane 1) gene replacement cassette consisting of the 5′ and 3′pyrG DNA inserts (without the coding region) with the size of ~3.6 kb and (b) genomic PCR product of the A. oryzae S1 wild type strain (lane 1) and its mutant derivative pyrGΔ0, strain GR6 (lane 2). Lane M: 1 kb DNA ladder (Fermentas, Canada).