The Scientific World Journal / 2013 / Article / Tab 1

Research Article

Development of a pyrG Mutant of Aspergillus oryzae Strain S1 as a Host for the Production of Heterologous Proteins

Table 1

Primers used in this study.

DesignationPrimer descriptionPrimer sequence

5′pyrpyrG 5′ region sense primer5′-GGCGCCACAATCGTTGAGGTA-3′
*revint-XhoIpyrG 5′ region antisense primer, the XhoI site is underlined5′-CTCGAGGTTGGCGATGGAGG-3′
*fwdint-XhoIpyrG 3′ region sense primer, the XhoI site is underlined5′-CTCGAGGTAGTGGTGGATACGTACT-3′
3′pyrpyrG 3′ region antisense primer5′-AAGCTTATCAGCTGCATATCTCTAT-3′
Pyr5′-outpyrG 5′ region sense primer outside the pyrG 5′ gene replacement cassette5′-ATTTAGTCATTCCTTTTGGCATC-3′
Pyr3′-outpyrG 3′ region antisense primer outside the pyrG 3′ gene replacement cassette5′-CTAATTTTAATGTCTTCCTTCAG-3′

revint-XhoI was used to amplify the targeting region downstream of the pyrG coding region.
*fwdint-XhoI was used to amplify the targeting region upstream of the pyrG coding region.