Chitinase Genes LbCHI31 and LbCHI32 from Limonium bicolor Were Successfully Expressed in Escherichia coli and Exhibit Recombinant Chitinase Activities

Figure 2

SDS-PAGE analysis of four different recombinant chitinases. (a) Intracellular recombinant chitinase inrCHI31; 1: control transformant BL21-pET52b; 2: control transformant BL21-pET52b induced by 1.0 mM IPTG; 3: protein marker; 4: transformant BL21-inCHI31; 5–8: transformant BL21-inCHI31 induced by 1.0 mM IPTG for 3, 4, 5, and 6 h. (b) Extracellular recombinant chitinase exrCHI31; 1–3: control transformant BL21-pET52b induced by 1.0 mM IPTG for 3, 6, and 0 h; 4, 9: protein marker; 5–8: transformant BL21-exCHI31 induced by IPTG for 3, 4, 5, and 6 h; 10-11: transformant BL21-exCHI31 was not induced. (c) Intracellular recombinant chitinase inrCHI32; 1–5: transformant BL21-inCHI32 induced by 1.0 mM IPTG for 6, 5, 4, 3, and 0 h; 6: control transformant BL21-pET52b induced by 1.0 mM IPTG for 5 h; 7: protein marker. (d) Extracellular recombinant chitinase exrCHI32. 1: protein marker; 2–5: transformant BL21-exCHI32 induced by 1.0 mM IPTG for 3, 4, 5, and 6 h; 6: protein marker; 7, 8: control transformant BL21-pET52b induced for 0 and 3 h at 30°C.