Chitinase Genes LbCHI31 and LbCHI32 from Limonium bicolor Were Successfully Expressed in Escherichia coli and Exhibit Recombinant Chitinase Activities
Figure 5
The activities of four different recombinant chitinases on various substrates. (A) Activities of recombinase inrCHI31 to various substrates; (B) activities of recombinase exrCHI31 to various substrates; (C) activities of recombinase inrCHI32 to various substrates; (D) activities of recombinase exrCHI32 to various substrates. One percent (w/v) of substrate was dissolved in 0.02 mol L−1 HAc-NaAc buffer (pH 4.5) as reaction solution. Subsamples of the reaction solution were collected and used to measure chitinase activity. Substrates: 1, N.O-carb-chitin; 2, colloidal chitin; 3, chitosan; 4, N.O-carb-chitosan; 5–10, cell wall chitin of S. sclerotiorum, F. oxysporum, R. solani, P. sojae, A. alternata, and V. sordida. The experiments were performed three times. Different letters above the columns indicated a significant difference determined by Duncan’s multiple comparisons test ().
