Genome-Wide Analysis of DNA Methylation in Human Amnion

Table 4

UCN and OXTR promoter methylation status in the amnion and decidua from term (TNL, TL) and preterm (PTL) deliveries¹.

UCN
Site^2	Amnion				Decidua
	TL		PTL	𝑃-value	TL		PTL	𝑃-value
-190^3	2.2%		8.6%	0.39	6.7%		14.8%	0.07
-279^3	2.2%		11.4%	0.07	15.6%		12.5%	0.97
-319^4	24.4%		22.9%	0.83	19.9%		30.8%	0.08
-335^4	23.3%		27.1%	0.56	23.2%		25.8%	0.75
-361^4	48.9%		55.7%	0.33	32.1%		36.4%	0.48
OXTR
Site^2	Amnion				Decidua
	TNL	TL	PTL	𝑃-value	TNL	TL	PTL	𝑃-value
-860	24.4%	22.2%	24.3%	0.96	20%	15.6%	24.6%	0.19
-901	37.8%	30%	45.7%	0.17	45.6%	38.9%	46.9%	0.66
-924	56.7%	62.2%	68.6%	0.29	60%	52.2%	67.2%	0.09
-934^5	50%	41.1%	59.3%	0.22	46.7%	42.2%	57.1%	0.02
-959^5	43.3%	24.4%	27.1%	0.014	33.3%	33.3%	30.6%	0.91
-1084^5	4.4%	4.4%	5.7%	0.97	10%	0%	8.7%	0.008

¹Presents average % methylation at each CpG site.
²Nucleotide positions relative to translation start site.
³CpG sites in UCN with the lowest 𝑃-value in each tissue type.
⁴CpG sites methylated at higher levels in both tissues than the average methylation level of all sites examined.
⁵CpG sites with statistically significant (𝑃<0.05) differential methylation in either tissue. Details of statistical tests used are described in Section 2.
Abbreviations: TNL: term no labor; TL: term labor; PTL: preterm labor.