An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum

Table 2

Summary of optimization results for Coriandrum sativum proteases in comparison to other proteases.

Optimization parameter	Optimum conditions for proteases
	Coriandrum sativum	Papain^a	Bromelain^b	Trypsin^c
Enzyme concentration (mg/mL); Substrate concentration (mg/mL); Temperature (°C); Incubation time (min); pH	0.45; 0.425; 35; 20; 8–9.5	0.1; 0.1; 30; 30; 5–7	0.1; 0.25; 40; 30; 5-6	0.1; 0.1; 40; 30; 5–7

aShukor et al. [8].
bShukor et al. [10].
cShukor et al. [11].