Research Article

Comparative Studies on Cellular Behaviour of Carnation (Dianthus caryophyllus Linn. cv. Grenadin) Grown In Vivo and In Vitro for Early Detection of Somaclonal Variation

Table 2

Comparison between mitotic index (MI) of in vivo and in vitro grown Dianthus caryophyllus root meristems.

Dianthus caryophyllus MS media + hormone
(mg L−1)
AgeMitotic index, MI
(Percentage, %)

In vivo 4-day-old
(standard growth)
43.51 ± 2.14f

In vitro 2 mg L−1  NAA1-day-old41.20 ± 0.79e
2-day-old40.23 ± 1.30de
3-day-old37.35 ± 0.40c
4-day-old32.32 ± 1.55a
1-week-old39.20 ± 1.54cd
2-week-old38.67 ± 1.35cd
3-week-old43.77 ± 2.33f
4-week-old38.62 ± 1.75cd
5-week-old38.20 ± 3.24c
6-week-old37.31 ± 1.27c
7-week-old37.69 ± 0.66c
8-week-old38.29 ± 1.87c
3-month-old31.83 ± 0.81a
4-month-old34.45 ± 0.70b
6-month-old33.17 ± 0.78ab

Means with different letters in the same column differ significantly at P < 0.05 by one-way ANOVA and Duncan’s multiple range test.