Comparative Studies on Cellular Behaviour of Carnation (Dianthus caryophyllus Linn. cv. Grenadin) Grown In Vivo and In Vitro for Early Detection of Somaclonal Variation

Table 3

Comparison between chromosome numbers of in vivo and in vitro grown Dianthus caryophyllus root meristems.

Dianthus caryophyllus	MS media + hormone (mg L^−1)	Age	Chromosome number (mean)
In vivo	—	4-day-old; (standard growth)	29.73 ± 0.12^a
In vitro	2 mg L^−1  NAA	1-day-old	29.40 ± 0.31^a
		2-day-old	28.87 ± 0.29^a
		3-day-old	29.67 ± 0.16^a
		4-day-old	29.00 ± 0.48^a
		1-week-old	29.47 ± 0.40^a
		2-week-old	29.87 ± 0.24^a
		3-week-old	29.93 ± 0.42^a
		4-week-old	29.53 ± 0.51^a
		5-week-old	28.80 ± 0.39^a
		6-week-old	28.73 ± 0.44^a
		7-week-old	28.67 ± 0.36^a
		8-week-old	27.93 ± 0.43^a
		3-month-old	28.27 ± 0.60^a
		4-month-old	28.31 ± 0.69^a
		6-month-old	29.00 ± 0.60^a

∗Means with different letters in the same column differ significantly at P < 0.05 by one-way ANOVA and Duncan’s multiple range test.