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The Scientific World Journal
Volume 2013, Article ID 689235, 6 pages
http://dx.doi.org/10.1155/2013/689235
Research Article

Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates

1Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia
2Department of Animal Production, Faculty of Agriculture, University of Sabha, Sabha, Libya
3Institute of Tropical Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia
4Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, UPM, 43400 Serdang, Malaysia
5Institute of Bioscience, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia
6Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia

Received 30 August 2013; Accepted 26 September 2013

Academic Editors: M. Talat and N. Vassilev

Copyright © 2013 Mohamed Idris Alshelmani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the optimum moisture ratio required for solid state fermentation (SSF) of palm kernel cake (PKC). The bacteria cultures were grown on reconstituted nutrient broth, incubated at 30C and agitated at 200 rpm. Carboxymethyl cellulase, xylanase, and mannanase activities were determined using different substrates and after SSF of PKC. The SSF was conducted for 4 and 7 days with inoculum size of 10% (v/w) on different PKC concentration-to-moisture ratios: 1 : 0.2, 1 : 0.3, 1 : 0.4, and 1 : 0.5. Results showed that Bacillus amyloliquefaciens 1067 DSMZ, Bacillus megaterium 9885 ATCC, Paenibacillus curdlanolyticus 10248 DSMZ, and Paenibacillus polymyxa 842 ATCC produced higher enzyme activities as compared to other bacterial cultures grown on different substrates. The cultures mentioned above also produced higher enzyme activities when they were incubated under SSF using PKC as a substrate in different PKC-to-moisture ratios after 4 days of incubation, indicating that these cellulolytic bacteria can be used to degrade and improve the nutrient quality of PKC.