Characterization of Human Colorectal Cancer MDR1/P-gp Fab Antibody
Construction and verification of the P-gp recombinant. (a) The total RNA of human colorectal cancer, M: DL2000 DNA marker; lane 1: total RNA of human colorectal cancer. (b) PCR amplification of P-gp21 gene, M: DL2000 DNA Marker; lane 1: blank control; lane 2: target gene of P-gp21. (c) pET28a (+) and P-gp21 gene digested by EcoR I/Xho I double enzyme, M: DL10000 DNA Marker; lane 1: the digested pET28a (+); lane 2: the digested P-gp21 target gene. (d) Positive clone of P-gp21 verified by double-enzyme cleavage, M: DL10000 DNA Marker; lane 1: positive clone of P-gp21.