Characterization of Thermo- and Detergent Stable Antigenic Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham. and Evaluation of Its Ecofriendly Applications
Table 2
Effect of various organic solvents on proteolytic activity.
Sr. no.
Solvent
Residual activity (%)
Concentration of organic solvent (v/v)
30%
50%
70%
1
Control
100.0
100.0
100.0
2
Acetone
100 ± 0.43
98.22 ± 0.65
87.21 ± 0.13
3
Acetophenone
69.89 ± 4.35
87.84 ± 1.86
64.54 ± 1.46
4
Benzyl alcohol
80.15 ± 1.44
70.68 ± 0.27
42.44 ± 0.66
5
Benzene
95.15 ± 3.83
98.80 ± 3.57
92.19 ± 2.72
6
Butanol
63.96 ± 3.46
54.68 ± 3.56
31.68 ± 3.34
7
Chloroform
65.05 ± 2.86
57.55 ± 2.85
26.28 ± 2.35
8
Chlorobenzene
81.43 ± 2.85
100 ± 1.82
71.57 ± 2.16
9
Dichloromethane
58.52 ± 2.35
61.20 ± 2. 24
34.29 ± 2.37
10
Dimethylformamide
98.71 ± 5.97
90.13 ± 3.09
58.63 ± 3.38
11
Diethyl ether
50.16 ± 6.05
75.91 ± 4.78
61.39 ± 4.25
12
Ethylene glycol
100 ± 1.39
73.63 ± 3.06
38.14 ± 4.32
13
Ethy lacetate
58.63 ± 7.85
66.83 ± 5.85
45.51 ± 4.35
14
Methanol
71.18 ± 0.96
77.59 ± 1.75
66.83 ± 1.36
15
Propanol
84.11 ± 1.70
69.89 ± 1.58
52.11 ± 1.69
16
Tetrahydrofuran
70.37 ± 4.81
52.50 ± 7.95
18.36 ± 5.85
17
Trichloroethylene
60.81 ± 2.05
80.15 ± 2.90
70.68 ± 2.37
Data represented in average values ± SD of experiment.
