High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures
Figure 2
Cyclic somatic embryogenesis from germinated SEs on liquid medium. (a) Secondary SEs (bright white) induced directly from primary germinated SEs (black) in liquid medium 4 weeks after being cultured in MS liquid medium without PGRs. (b) Collected embryogenic cell clumps induced from germinated SEs in liquid medium. (c) Numerous heart-shaped SEs which developed after embryogenic calluses were cultured in MS liquid medium without PGRs for 6 weeks. (d) Cotyledonary SEs after embryogenic calluses were cultured in liquid medium for 8 weeks. (e) Germinated SEs after embryogenic calluses were cultured in liquid medium for 10 weeks. (f) Plantlets from germinated SEs on agar medium. Bars = 10 mm (a), 15 mm (b), 10 mm (c), 10 mm (d), 15 mm (e), and 10 mm (f).
