Preamplification Procedure for the Analysis of Ancient DNA Samples

Table 1

List of primers and probes. For each target name, sequence, concentration used, amplicon size, and reference are shown.

Target	Primer and probe names	5′ Sequence	Concentration (nM)	Amplicon size (bp)	Ref.
Soy lectin	GM1-F; GM1-R; Probe GM1	CCAGCTTCGCCGCTTCCTTC; GAAGGCAAGCCCATCTGCAAGCC; FAM-CTTCACCTTCTATGCCCCTGACAC-TAMRA	600; 600; 120	74	[20, 32]
Human; mtDNA	mt coding-F; mt coding-R; Probe coding	CCAACACCTCTTTACAGTGAAATGC; GTGATGAGGAATAGTGTAAGGAGTATGG; FAM-CAACTAAATACTACCGTATGGC-MGB	300; 300; 200	92	[22] This work
Human; mtDNA; standard	mt cod 8200F; mt cod 8559R	GTTTCATGCCCATCGTCCTA; GCAATGAATGAAGCGAACAG	100; 100	360	This work
Human; AMG X/Y	AMG-F; AMG-R; Probe AMG-X; Probe AMG-Y	CCCTGGGCTCTGTAAAGAATAGTG; ATCAGAGCTTAAACTGGGAAGCTG; FAM-TATCCCAGATGTTTCTC-MGB; VIC-CATCCCAAATAAAGTG-MGB	100; 100; 150; 150	106/112	[2]