Preamplification Procedure for the Analysis of Ancient DNA Samples

Table 2

Summary of quantitative and qualitative analysis. Mitochondrial and nuclear DNA copy number and AMG and CFTR typing data of nonpreamplified and preamplified DNA extracted from the nine bone samples.

Sample	Bone type	Age	mtDNA (copies/𝜇L)		AMG X (copies/𝜇L)		AMG X/Y typing		CFTR typing
			Nonpreamplified	Preamplified	Nonpreamplified	Preamplified	Nonpreamplified	Preamplified	Nonpreamplified	Preamplified
B1	Carpal bone	2nd century B.C.–; 8th century A.D	<10	1.2×10^4	—	—	—	—	—	—
B2	Carpal bone		<10	7.6×10^5	—	5.8×10^5	—	XX	—	AG
B4	Carpal bone		10	1.1×10^4	—	—	—	—	—	—
B5	Long bone fragment		<10	8×10^3	—	1.4×10^3	—	XX	—	AA
B7	Cranial bone fregment		13	6.3×10^4	—	6.7×10^4	—	XY	—	AA
B9	Cranial bone fregment		<10	2×10^3	—	—	—	—	—	GG
A-1V	Vertebra	13–16th century A.D	9.3×10^2	3×10^6	2.5×10^2	3.4×10^5	XX	XX	—	AG
A-2	Cranial bone fregment		<10	6×10^3	—	—	—	Y	—	—
A-3	Cranial bone fregment		<10	1×10^3	—	—	—	—	—	—