The Scientific World Journal / 2013 / Article / Fig 2

Research Article

Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis

Figure 2

Morphological features and expression patterns of Isl1 and other cell differentiation markers in the St53 Xenopus laevis central retina. (a)–(c) DAPI fluorescence combined with Isl1 immunofluorescence. DAPI staining showed well-organized retinal layers. Isl1 expressing cells in the INL could be characterized morphologically and topographically as horizontal (arrowheads), bipolar (arrows), or amacrine cells (double arrows). Notice that not all cells located in the GCL expressed Isl1 (asterisks). (d)–(f) A few Isl1-positive bipolar cells also expressed CB (arrows). (g)–(i) Subpopulations of Isl1-positive horizontal (arrowheads), bipolar (arrows), and ganglion cells (double arrowheads) also expressed CR. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer. Scale bars: 100 μm.
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