Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis
Morphological features and expression patterns of Isl1 and other cell differentiation markers in the St40 Xenopus laevis retina. (a) Toluidine blue-stained transverse retinal resin sections revealed that the typical multilayered structure was clearly distinguishable, showing morphological features of maturation. The CMZ was visible in the peripheralmost region. (b) Isl1 immunoreactivity was clearly established in abundant nuclei located in the GCL, whereas the INL contained Isl1 labeling that corresponded to horizontal (arrowhead), bipolar (arrow), and amacrine cells (double arrowhead). (c) SV2 expression in the plexiform layers extended to more peripheral regions. (d)–(f) The CERN-922 labeling in the ONL coursed in parallel with Isl1 immunoreactivity and reached the periphery by this stage. CMZ: ciliary marginal zone; GCL: ganglion cell layer; INL: inner nuclear layer; L: lens; ONH: optic nerve head; ONL: outer nuclear layer. Scale bars: 100 μm.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.