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The Scientific World Journal
Volume 2013, Article ID 781282, 5 pages
Research Article

Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR

1Department of Plant Production and Technologies, Faculty of Agricultural Sciences and Technologies, University of Niğde, 51240 Niğde, Turkey
2Department of Biology, Faculty of Arts and Science, University of Osmaniye Korkut Ata, 80000 Osmaniye, Turkey
3Roche Diagnostics, 34394 Istanbul, Turkey
4Department of Horticulture, Faculty of Agriculture, University of Çukurova, 01330 Adana, Turkey

Received 17 March 2013; Accepted 9 May 2013

Academic Editors: M. Aasim, A. Bakhsh, K. M. Khawar, S. Onarici, C. A. Ozel, and A. Q. Rao

Copyright © 2013 Hatıra Taşkın et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was performed for comparison of meristem culture technique with shoot tip culture technique for obtaining virus-free plant, comparison of micropropagation success of two different nutrient media, and determination of effectiveness of real-time PCR assay for the detection of viruses. Two different garlic species (Allium sativum and Allium tuncelianum) and two different nutrient media were used in this experiment. Results showed that Medium 2 was more successful compared to Medium 1 for both A. tuncelianum and A. sativum (Kastamonu garlic clone). In vitro plants obtained via meristem and shoot tip cultures were tested for determination of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV) through real-time PCR assay. In garlic plants propagated via meristem culture, we could not detect any virus. OYDV and LYSV viruses were detected in plants obtained via shoot tip culture. OYDV virus was observed in amount of 80% and 73% of tested plants for A. tuncelianum and A. sativum, respectively. LYSV virus was found in amount of 67% of tested plants of A. tuncelianum and in amount of 87% of tested plants of A. sativum in this study.