¹⁸F-Fluorothymidine-Pet Imaging of Glioblastoma Multiforme: Effects of Radiation Therapy on Radiotracer Uptake and Molecular Biomarker Patterns

Figure 6

Radiation therapy leads to marked increase in DNA damage within the targeted tumor. (a) H&E stain (1x) and immunofluorescent staining w/ DAPI (blue) for Gamma-H2AX (𝛾H2AX) (red) (4x and 20x) in control flank tumors, with specific focus on tumor core (white arrows). (b) Staining as in (a) in flank tumors after 16 Gy RT, with specific focus on the tumor core (yellow arrows) and notable 𝛾H2AX expression at 20x (blue arrows).

(a) Control 

(b) RT