Research Article

Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy

Figure 1

Abstract system model and temporal parameters. ( shows laser pulse, stands for sample lifetime, and means gate width of gated optical intensifier (GOI), , , , and represent number of gates opened in one excitation, gate interval of gates, delay step of different excitations, number of excitations, and the interval between laser excitation and opening of the first gate, resp.).
801901.fig.001a
(a) Time-gated FLIM principles
801901.fig.001b
(b) Abstract model of time-gated FLIM systems