Optimization of Extraction Parameters by Using Response Surface Methodology, Purification, and Identification of Anthocyanin Pigments in Melastoma malabathricum Fruit
Table 2
Adequecy of model tested.
Source
Sum of squares
df
Mean square
Value
Prob >
Remarks
Sequential model sum of squares for acidified methanolic extraction
Mean versus Total
20893255.04
1
20893255.04
Linear versus Mean
128877.78
3
42959.26
20.25
<0.0001
2FI versus Linear
10748.42
3
3582.81
2.13
0.1600
Quadratic versus 2FI
12433.06
3
4144.35
6.60
0.0189
Suggested
Cubic versus Quadratic
874.80
3
291.60
0.33
0.8045
Aliased
Residual
3520.73
4
880.18
Total
21049709.83
17
1238218.23
Sequential model sum of squares for acidified ethanolic extraction
Mean versus Total
7196887.80
1
7196887.80
Linear versus Mean
147605.80
3
49201.93
28.80
<0.0001
Suggested
2FI versus Linear
532.96
3
177.65
0.08
0.9683
Quadratic versus 2FI
13908.91
3
4636.30
4.18
0.0544
Suggested
Cubic versus Quadratic
6069.16
3
2023.05
4.76
0.0829
Aliased
Residual
1699.43
4
424.86
Total
7366704.07
17
433335.53
Source
Std. Dev.
Adjusted
Predicted
PRESS
Remarks
Model summary statistics for acidified methanolic extraction
Linear
46.058
0.824
0.783
0.654
54157.757
2FI
41.023
0.892
0.828
0.542
71629.308
Quadratic
25.059
0.972
0.936
0.875
19498.004
Suggested
Cubic
29.668
0.977
0.910
+
Aliased
Model summary statistics for acidified ethanolic extraction
Linear
41.334
0.869
0.839
0.741
43972.027
Suggested
2FI
46.559
0.872
0.796
0.406
100897.766
Quadratic
33.314
0.954
0.895
0.413
99761.992
Suggested
Cubic
20.612
0.990
0.960
+
Aliased
+ Case(s) with leverage of 1.0000: PRESS statistic not defined.
