Evaluation of the Antibacterial and Antifungal Potential of Peltophorum africanum: Toxicological Effect on Human Chang Liver Cell Line

Table 4

Lethal dose at 50 percent reduction of the cell population.

Dose (𝜇g/mL)	Probit percent			Actual percent*			LD50 (𝜇g/mL)*
	24 hrs	48 hrs	72 hrs	24 hrs	48 hrs	72 hrs	24 hrs	48 hrs	72 hrs
5	25.24	4.34	1.37	19.23	3.87	5.21	—	—	—
25	31.51	18.80	13.75	29.48	10.68	5.34	—	—	—
75	38.36	37.41	37.02	34.96	24.65	16.96	82.64±1.40	—	—
125	45.59	47.66	50.88	55.96	65.03	70.68	—	140.09±1.33	121.07±0.92
250	51.03	61.70	69.21	60.12	71.17	79.41	—	—	—
500	62.54	74.33	83.68	61.85	72.16	82.88	—	—	—
1000	77.35	84.37	92.80	70.98	80.86	88.43	—	—	—

∗LD₅₀(50% of the cells have been killed); actual and probit percents were calculated using probit statistical analysis software “NCSS 2007”; *actual % = actual formulas (𝑛 is the number of cells in a group); —: not applicable.