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The Scientific World Journal
Volume 2013, Article ID 891581, 7 pages
Research Article

Association of nNOS Gene Polymorphism with Ischemic Stroke in Han Chinese of North China

1Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang 110001, China
2Department of Neurology, NO 202 Hospital of People’s Liberation Army of China, Shenyang 110001, China
3Department of Neurology, Jinqiu Hospital of China Liaoning Province, Shenyang 110001, China

Received 30 June 2013; Accepted 30 July 2013

Academic Editors: W. Hall and R. I. Sakakibara

Copyright © 2013 Yingjie Dai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Nitric oxide (NO) is an important messenger molecule and effector molecule. This study aimed to investigate the relation of neuronal nitric oxide synthase (nNOS) gene polymorphism with ischemic stroke in Han Chinese of North China. This was a case-control study. A total of 413 patients with ischemic stroke were recruited from Han Chinese of North China. There were 201 males and 212 females. In addition, 477 healthy subjects served as controls including 224 males and 253 females. Multiplex SNaPshot was employed to detect nNOS gene polymorphism (rs2293050, rs2139733, rs7308402, and rs1483757). Results showed that the rs1483757, rs2139733, and rs2293050 genotypes and allele frequencies were comparable between patients and controls. However, ischemic stroke patients had significantly reduced AG genotype and A allele frequency when compared with controls ( , ). After adjusting confounding factors (gender, age, smoking, history of drinking, hypertension, and diabetes), AG genotype and A allele were still related to ischemic stroke ( , 95% CI: 0.335–0.978, ; , 95% C: 0.378–0.985, and ) and both were found to be protective factors. Our results showed that rs7308402 gene polymorphism of nNOS is related to ischemic stroke in Han Chinese of North China.