Application of Response Surface Methodology for Optimizing Arginine Deiminase Production Medium for Enterococcus faecium sp. GR7

Table 6

Regression analysis (ANOVA) for ADI specific activity.

Source	Sum of squares	Degree of freedom	Mean squares	F value	P value	Prob > F
Model	32.586	9	3.621	7.749	0.0018	Significant
A-tryptone	6.692	1	6.692	14.321	0.0036
B-lactose	1.163	1	1.163	2.489	0.1457
C-arginine	4.043	1	4.043	8.653	0.0147
AB	11.249	1	11.249	24.074	0.0006
AC	0.545	1	0.545	1.166	0.3056
BC	4.673	1	4.673	10.001	0.0101
A^∧2	4.030	1	4.030	8.625	0.0149
B^∧2	0.314	1	0.314	0.671	0.4317
C^∧2	0.225	1	0.225	0.481	0.5038
Residual	4.673	10	0.467
Lack of fit	4.626	5	0.925	99.432	<0.0001	Significant
Pure error	0.047	5	0.009
Cor total	37.258	19

Coefficient of determination (𝑅²) = 0.874.