Dengue Virus Type 2: Protein Binding and Active Replication in Human Central Nervous System Cells
Binding of dengue virus type 2 (DENV-2) to membrane proteins of the central nervous system (CNS). To establish which proteins in the human CNS were involved in binding DENV, VOPBA were carried out by using the entire DENV particles precipitated with polyethylene glycol and membrane proteins obtained from either white or gray matter from human tissue. Three membrane proteins (60, 70, and 130 kDa) from gray matter were found to bind the entire viral particle, while only one of them (70 kDa) was so identified in the white matter. MW: molecular weight; Vero: membrane proteins from Vero cells; NMB: membrane proteins form neonatal mouse brain; GM: membrane proteins from gray matter; WM: membrane proteins from white matter; kDa: kilodaltons. Arrows point to the proteins detected to bind virus particles in this assay. (b); (a) shows proteins in SDS-PAGE 12%.
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