The Scientific World Journal / 2013 / Article / Tab 4 / Research Article
Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor Table 4 Central composite design matrix (coded) and result for the model of TEA-based esterquat synthesis.
Run no. Enzyme amount (w/w%)
Reaction time (hour)
Reaction temperature (°C)
Molar ratio of substrates (mole)
Agitation speed (r.p.m.) Conversion % Actual Predicted 1 0 0 0 0 0 56.44 53.56 2 0 0 0 0 −1.75 47.78 43.86 3 −1 1 −1 −1 1 48.22 52.69 4 −1 −1 −1 −1 1 46.44 47.63 5 1 −1 1 −1 1 57.56 57.28 6 −1 1 1 −1 1 63.56 65.25 7 1 −1 −1 −1 −1 48.89 48.17 8 −1 1 1 −1 −1 62.89 62.84 9 1 −1 1 1 1 50.67 48.33 10 −1 1 −1 1 1 44.00 43.07 11 0 0 −1.75 0 0 39.56 41.75 12 −1 −1 −1 1 1 32.00 30.96 13 0 0 0 0 0 51.11 53.56 14 −1 −1 −1 −1 −1 44.44 45.23 15 −1 −1 1 −1 1 57.78 54.33 17 0 1.75 0 0 0 60.00 60.65 18 1 −1 −1 1 −1 25.00 25.53 19 1 −1 −1 −1 1 52.67 50.58 20 −1 −1 −1 1 −1 25.56 22.59 21 0 0 0 0 1.75 46.22 53.29 22 1 1 1 −1 −1 63.33 65.78 23 −1 1 −1 −1 −1 52.22 50.27 24 1 −1 −1 1 1 33.78 33.90 25 1.75 0 0 0 0 48.44 50.48 26 0 0 0 1.75 0 38.00 42.84 27 1 1 −1 1 −1 35.33 37.64 28 1 1 −1 −1 1 59.11 55.62 29 −1 −1 1 −1 −1 54.44 51.92 30 0 0 0 0 0 62.44 53.56 31 1 1 1 1 −1 58.00 57.94 32 0 0 0 0 0 58.00 53.56 33 0 0 0 0 0 55.33 53.56 34 −1 1 1 1 1 64.89 63.37 35 −1 −1 1 1 1 44.89 45.38 36 −1 1 −1 1 −1 34.89 34.70 37 1 1 1 1 1 67.11 66.32 38 1 1 −1 −1 −1 52.67 53.22 39 1 1 1 −1 1 71.33 68.19 40 −1.75 0 0 0 0 44.22 45.32 41 1 −1 1 −1 −1 48.89 54.87 43 0 0 0 0 0 46.89 53.56 44 0 0 0 0 0 52.00 53.56 45 −1 1 1 1 −1 54.22 55.00 46 1 −1 1 1 −1 41.33 39.96 47 −1 −1 1 1 −1 35.78 37.02 48 0 0 0 0 0 53.11 53.56 49 0 −1.75 0 0 0 38.00 40.50 50 1 1 −1 1 1 49.33 46.01