Agrobacterium tumefaciens-Mediated Transformation of Valsa mali: An Efficient Tool for Random Insertion Mutagenesis

Table 1

Comparison of some important biological properties and pathogenicity between transformants and wild-type strain LXS240101.

Strain	Colony diameter; (cm)	Sporulation quantity; (×10^6 spores/plate)	Spore germination; ratio (%)	Lesion areas; (cm^2)
LXS240101	8.78±0.45	11.50±2.06	92.18±1.34	14.66±3.54/13.87±2.98^a
PFL1	7.86±0.67	0.00±0.00^∗	—	13.15±3.40
PFL2	3.99±0.68^∗	0.75±0.36^∗	94.95±1.91	5.54±1.97^∗
PFL3	6.56±0.26^∗	12.06±3.42	86.52±8.96	11.99±1.89
PFL4	8.84±0.11	16.94±4.05	88.82±9.33	3.47±0.58^∗
PFL5	8.03±0.36	240.00±35.18^∗∗	59.22±9.82^∗	1.23±0.07^∗
PFL6	5.62±0.16^∗	0.13±0.28^∗	0.00±0.00^∗	2.65±0.43^∗
PFL7	8.84±0.39	11.25±4.74	81.93±11.13	8.93±4.18
PFL8	8.27±0.35	23.75±11.57	81.73±10.42	8.01±2.27
PFL9	8.31±0.99	116.88±22.49^∗∗	22.93±5.23^∗	1.64±0.67^∗
PFL10	8.18±0.34	15.00±5.14	86.92±7.33	1.43±0.56^∗
PFL11	4.27±0.25^∗	2.75±0.81^∗	75.02±14.48	13.02±2.30
PFL12	8.24±0.54	150.00±60.11^∗∗	51.88±9.75^∗	1.35±0.65^∗
PFL13	5.79±0.25^∗	0.00±0.00^∗	—	1.79±0.43^∗
PFL14	8.23±0.13	37.50±13.87	83.62±6.82	8.30±1.80
PFL15	8.58±0.48	0.00±0.00^∗	—	10.24±0.91
PFL16	8.71±0.35	20.63±9.22	13.32±4.54^∗	2.21±0.46^∗
PFL17	7.28±0.56^∗	0.00±0.00^∗	—	4.70±1.53^∗
PFL18	9.00±0.00	26.31±5.65	90.54±3.43	23.18±0.80^∗∗
PFL19	6.63±0.22^∗	23.44±14.04	17.49±9.29^∗	4.94±0.38^∗
PFL20	8.26±0.35	15.94±2.59	92.10±2.49	13.35±0.91
PFL21	8.16±0.57	25.00±4.74	9.88±1.10^∗	4.98±0.11^∗
PFL22	7.83±0.78	18.75±2.05	85.57±5.26	12.57±3.55
PFL23	6.69±0.22^∗	156.88±40.47^∗∗	85.82±4.12	7.82±4.49
PFL24	8.30±0.36	7.50±3.56	85.15±11.19	10.39±1.25
PFL25	7.94±0.54	110.63±24.27^∗∗	57.82±9.01^∗	8.17±2.29
PFL26	8.24±0.17	9.50±2.97	94.87±3.78	3.63±1.32^∗
PFL27	8.05±0.42	387.50±68.89^∗∗	69.95±13.33^∗	4.98±0.98^∗
PFL28	8.54±0.28	13.56±3.11	88.66±9.03	11.11±0.47
PFL29	8.72±0.07	34.38±12.62	62.00±14.03^∗	7.18±3.98
PFL30	7.36±0.11^∗	17.13±4.13	89.34±5.28	1.88±0.38^∗

Duncan’s multiple range test between wild-type strain LXS240101 and its transformants, respectively; aindicated lesion areas caused by conidia suspensions/mycelium plug; ∗indicated significantly decreased values for transformants at 𝑃_0.05; ∗∗indicated significantly increased values for transformants; —: indicated that the trial was not conducted.