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The Scientific World Journal
Volume 2014, Article ID 125353, 8 pages
http://dx.doi.org/10.1155/2014/125353
Research Article

Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48

1Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia
2Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia

Received 11 May 2014; Accepted 2 July 2014; Published 24 July 2014

Academic Editor: Chen Yao

Copyright © 2014 Mohd Zabidi Majid et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Description for Supplementary 1: Cells undergoing apoptotic activity eventually produces fragments of dense granular particles due to the activities of endogenous nucleases. These apoptotic bodies are easily stained green fluorescent using the IHC technique. Actinomycin D is an alkylating agent that induces apoptosis of cancer cells and was thus used as a positive control in the study.

Description for Supplementary 2 & 3: Apoptosis of cancer cells resulted from DNA fragmentation of the chromatin into nucleosomal units. When run on agarose gel electrophoresis these units appear as DNA ladder. Thus, determining whether a cell exhibits DNA fragmentation can provide information about the mode of cell death induced by the respective extracts.

  1. Supplementary Material