Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest

Figure 9

(a) AAA induced activation of intrinsic apoptotic pathway in COLO-205 cells. Agarose gel electrophoresis of PCR products of (A) β-actin, (B) caspase-9, (C) caspase-3, (D) Bax, and (E) Bcl-2 genes. (b) The fold change in expression of apoptosis-associated gene after AAA treatment. The densitometric analysis was done of the PCR products of caspase-9, caspase-3, Bax, and Bcl-2 genes to measure the fold change in each gene and was normalised to the house-keeping gene β-actin. Data presented as fold change in gene expression between AAA treated cells and untreated control cells. ; .

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