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The Scientific World Journal
Volume 2014 (2014), Article ID 129697, 15 pages
Research Article

Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest

Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh 173234, India

Received 23 July 2014; Accepted 28 August 2014; Published 27 October 2014

Academic Editor: David E. Misek

Copyright © 2014 Shagun Arora and Simran Tandon. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material: To assess the cytotoxic effects of aqueous(AAA) and ethanolic(EAA) extracts of Achyranthes Aspera Root on human colon cancer cells (COLO-205) various assays were carried out.

The results revealed that both the extracts were able to reduce the viability in a dose dependent manner although AAA had more pronounced effects (Figure S1).These results were qualitatively verified by AO/EB(Figure S2) and Annexin V/PI(Figure S3) staining which revealed the ability of both the extracts to induce apoptosis in the COLO-205 cells, whereas the untreated cells showed no evidence of apoptosis.

The data generated revealed that in comparison to EAA, AAA showed more promise in terms of eliciting cell death. Therefore this extract was pre-treated with β-glucosidase to mimic in vivo conditions and it was seen that enhanced cytotoxicity to the COLO-205 was observed as compared to the extract in which no pre-treatment with the enzyme had been carried out(S4). These results indicated that the AAA could be modified by enzymes present in vivo leading to greater toxicity to the cancer cells.

Finally GC-MS analysis of AAA revealed the presence of 10 biological compounds which could be responsible for the various effects seen on COLO-205 cells (S5).

  1. Supplementary Material