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The Scientific World Journal
Volume 2014, Article ID 132308, 9 pages
http://dx.doi.org/10.1155/2014/132308
Research Article

Cell Adhesion to Plasma-Coated PVC

1Laboratório de Plasmas Tecnológicos, Universidade Estadual Paulista (UNESP), Avenida Três de Março 511, 18087-180 Sorocaba, SP, Brazil
2Laboratório de Biomateriais, Departamento de Ciências Fisiológicas, Pontifícia Universidade Católica, Praça Dr. José Ermírio de Moraes 290, 18030-095 Sorocaba, SP, Brazil
3Laboratório de Superfícies e Interfaces, Departamento de Física, Universidade Federal do Paraná, 81531-990 Curitiba, PR, Brazil

Received 27 March 2014; Revised 12 August 2014; Accepted 14 August 2014; Published 26 August 2014

Academic Editor: Raju Adhikari

Copyright © 2014 Elidiane C. Rangel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To produce environments suitable for cell culture, thin polymer films were deposited onto commercial PVC plates from radiofrequency acetylene-argon plasmas. The proportion of argon in the plasmas, PAr, was varied from 5.3 to 65.8%. The adhesion and growth of Vero cells on the coated surfaces were examined for different incubation times. Cytotoxicity tests were performed using spectroscopic methods. Carbon, O, and N were detected in all the samples using XPS. Roughness remained almost unchanged in the samples prepared with 5.3 and 28.9% but tended to increase for the films deposited with PAr between 28.9 and 55.3%. Surface free energy increased with increasing PAr, except for the sample prepared at 28.9% of Ar, which presented the least reactive surface. Cells proliferated on all the samples, including the bare PVC. Independently of the deposition condition there was no evidence of cytotoxicity, indicating the viability of such coatings for designing biocompatible devices.