Using Targeted Virotherapy to Treat a Resistant Ewing Sarcoma Model: From the Bedside to the Bench and Back
Figure 3
VSVΔM51 infects and kills EWS cells established from the patient’s tumor specimen. EWS cells were inoculated with VSVΔM51-RFP at MOI 0.01. Viral replication was assessed at 24 h and 48 h by fluorescent microscopy for RFP expression. Light microscopy was used to assess cell death after infection. Robust infection was noted at 24 h and massive cell death was confirmed qualitatively by characteristic cytopathic rounding and detachment of cells off the culture dish at 48 h.
