Comparative Analysis of Cardiovascular Development Related Genes in Stem Cells Isolated from Deciduous Pulp and Adipose Tissue

Figure 1

(a-b) Morphology of SHED and ASC using phase contrast microscope at 10x magnification. In vitro mesoderm differential potential of SHED and ASC. Adipogenesis was detected by neutral oil droplet formation stained with oil red O. Chondrogenesis was detected by the presence of proteoglycans stained with Alcian Blue. Osteogenesis was confirmed by mineralized matrix deposition stained with von Kossa staining. All staining processes were done 21 days after induction for SHED (c–e) and ASC (f–h). (i-j) Immunophenotype analysis of SHED and ASC. Cells were tested against human antigens CD34, CD44, CD45, CD73, CD90, CD166, and HLA-DR. All experiments were conducted at passage 3 with 3 biological replicates for each established cell line. The percentages of positive cells shown in the figure are average of the three donors.

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