Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry

Table 2

Intrabatch and interbatch precision and accuracy of PD in rat plasma.

Nominal concentration; (ng/mL)	Measured concentration; (ng/mL)	CV; (%)	RE; (%)
Intrabatch^a
50	50.9±1.6	3.2	1.8
150	148.0±5.5	3.7	−1.3
1000	1007.3±44.2	4.4	0.7
8000	7913.0±411.6	5.2	−1.1
10000	10309.1±282.0	2.7	3.1
Interbatch^b
50	50.4±2.0	4.0	0.7
150	136.9±7.0	6.2	−8.7
1000	968.3±75.3	8.0	−3.2
8000	7877.1±693.6	8.8	−1.5
10000	10101.7±352.4	1.0	3.5

CV: coefficient of variation; RE: relative error.
aComparisons of five replicates (𝑛=5) observations at each concentration.
bComparisons of 25 replicates (𝑛=5) observations over five different analytical runs.