Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry

Table 3

Recovery and matrix effect of platycodin D and IS in rat plasma (𝑛=4 at each level).

Nominal concentration	Absolute matrix effect; (%)	Recovery; (%)
Platycodin D
150 ng/mL	86.2	94.5
1000 ng/mL	90.4	92.0
8000 ng/mL	99.3	90.4
Notoginsenoside R1 (IS)
1000 ng/mL	99.1	73.6

Absolute matrix effect was expressed as the ratio of the mean peak area of an analyte spiked into plasma extract after acetonitrile treatment to the mean peak area of the same analyte standards in mobile phase multiplied by 100.
Recovery was calculated as the ratio of the mean peak area of an analyte spiked into plasma before acetonitrile treatment to the mean peak area of an analyte spiked into plasma extract after acetonitrile treatment multiplied by 100.